DNA barcoding and morphometric analysis of oriental fruit fly Bactrocera occipitalis (Bezzi) and B. philippinensis (Drew and Hancock) (Diptera: Tephritidae) from Guimaras, Philippines.

Date

4-2012

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Co-adviser

Celia DR Medina

Abstract

DNA and morphometric analyses were done to compare variation between the oriental fruit flies Bacirocera occipitalis and B. philippinensis. Two base pair sequences (a 556- and 619 base pairs) found at the 5' end of the mitochondria) cytochrome c oxidase I gene were used. Sequence alignment for both primer sets indicated high sequence similarity (96-100% homology). Construction of neighbor-joining trees showed that genetic distance ranged from 0-0.161% for the 556 base-pair sequences and 0-0.014% for the 619 base pair sequences. Morphometric analyses of the scutellum width, scutellum length, femur, tibia, and tarsus lengths of the fore-, mid-, and hindlegs, lengths of antenna) segments, and distances between lateral and medial ocelli showed no significant differences for B. occipitalis and B. philippinensis. Based on these results, B. occipitalis could not be delineated from B. philippinensis.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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