Electrophoretic analysis of soluble proteins and glucose-6- phosphate dehydrogenase isozymes in four accessions of Azolla pinnata variety pinnata

Date

10-1990

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Liwayway M. Engle

Co-adviser

Presidena A. Baculod

Abstract

Isozyme electrophoresis was used to investigate similarities and differences among four accessions of Azolla pinnata variety pinnata. Relative electrophoretic mobilities of soluble proteins revealed differences among the selected accessions of Azolla, pinnata variety pinnata. However, similar bands were also observed. Five bands were common to all the accessions. Relative electrophoretic mobilities of glucose-6-phosphate dehydrogenase also showed differences among accessions. Three isozymes were detected. All the accessions studied showed an A isozyme. Three accessions exhibited the B isozyme:. Moreover. one accession exhibited a unique band. Comparisons of A, pinnate with other species revealed differences in protein banding patterns. Protein bands of pinnata migrated faster as compared to the other species. On the other hand, glucose-6-phosphate dehydrogenase isozymes of A pinnata were generally slower. Moreover, intraspecific taxa of A. pinnate revealed higher similarities. Results obtained, indicated genetic differences among accessions of a pinnata and between Azalla species. Consistency in banding patterns of G-6-PD makes it a good biochemical marker for Azolla species and accessions.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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