Comparative electrophoretic analysis of the total protein of selected organs of tilapia Oreochromis niloticus (Linnaeus) from Biñan and Los Baños, Laguna

Date

4-1998

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

Adviser/Committee Chair

Ivan-Marcelo A. Duka

Abstract

Sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) was used to determine the number of total protein bands of the muscle, heart, eye and liver tissues of Oreochronus niloiicus (Linnaeus) from Binan and Los Banos, Laguna. There were a total of 22 muscle, 29 heart, 25 eye and 23 liver bands in the Binan samples. The Los Banos samples have a total of 19 muscle, 25 heart, 28 eye and 22 liver bands. Band numbers 26 and 27 of the eye and heart and band numbers 28 and 29 of the eye could probably serve as potential markers. No common banding pattern was detected within organs from the same locality and among organs in different localities. Furthermore, the comparison of the generalized banding patterns of the same organ from the two localities revealed marked differences due to the absence and presence of some protein bands. This is due to the significant differences in the number of total proteins among organs caused by the organ-environment interaction verified by the statistical tests. Despite these differences, the tilapia from the two sites have a considerable degree of homogeneity due to the high similarity index (SI) among the muscles (51.32%), the hearts (80.61) and the eyes (78.74).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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