Karyotype analysis of In Vitro cultured leukocytes treated with ampalaya tablets (Momordica charantia L.)

Date

5-1999

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Cells from Giemsa-stained slides of human male leukocytes cultured in vitro treated with different concentrations (0. 1, 3, and 5%) of ampalaya tablets were examined. The chromosomes were arranged in a karyotype and the relative lengths were calculated. Result showed that although treatments with ampalaya tablets caused observable changes in the chromosome length, it did not significantly change the relative lengths of the chromosomes. A correlation was done between the slope and the change in length. Chromosomes 2, 3, and 12. exhibited a continuous decrease in length as the concentration of the amplaya tablet increased. Chromosome 3 appeared the most condensed because it had the most negative slope. Other chromosomes showed a continuous increase in length as the concentration increased (e.g. chromosomes 1, 9, 13 and X). Chromosome 9 had the most positive slope. The rest of the chromosomes manifested a fluctuating reaction to the ampalaya tablet. Group A had the most negative slope while group D has the most positive slope. The results imply that the change in the length of the chromosomes could not be attributed to the ampalaya tablets.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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