A preliminary study on the effect of ampalaya (Momordica charantia L.) crude extracts on albino mice (Mus musculus) as measured by isozyme analysis

Greg C. Sigua

Date

3-1992

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

The effect of ampalaya (M. charantia) crude extracts on erythrocyte enzymes namely esterase, alkaline phosphatase, acid phosphatase, and G-6-PD and on fresh Maid of albino mice (Mum musculus) was determined using starch gel electrophoresis and PAGE respectively. No results were obtained for the isozymes of the mice killed 24 hones after treatment while for the total protein an extra band was seen for those treated with 30% and 60% w/v extract compared to that of the control. For the mice killed 14 Days after treatment no bands were observed for G-6-PD while ho variability was seen for acid and alkaline phosphatase. For esterase, out of two runs one mouse treated with 30% and 60% had one extra band. Since the extra band was not observed in all the treated mice it can not be said that the ,viability is definitely due to the extract. For the total protein, the mice treated with 30% and 60% had two extra bands compared to that of the control. The results indicate that M. charantia has an effect on gene action due to the differences in banding pattern observed in the total protein ,1 the treated and untreated mice killed 24 hours and 14 days after treatment and that the effect is manifested more at 14 days after.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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