Meiosis in seleceted Philippine indigenous orchids of tribe Vandeae

Date

3-2008

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

Meioses in four Philippine indigenous orchids of tribe Vandeae namely: Phalaenopsis philippinensis Golamco ex Fowlie & C. Z. Tang, Phalaenopsis schilleriana Rchb. f., Staurochilus ionosma (Lindl.) Schltr. and Vanda lamellata Lindl. were studied using the acctocarmine-squash technique. Young flower buds form different locations were fixed between 10 .m. to 12 noon in Farmer's solution. Chromosome number, meiotic chromosome behavior and pollen fertility were determined and compared. At Diakinesis, several chromosome configurations were observed in the four orchid species but all gave a total count of 38, therefore the diploid chromosome number is 2n=38. The most frequent configuration were 1811 + 21 in P. philippinensis (26.92%), 1611 + 61 and 1511+ 81 in P. schilleriana (22.95% for both), 1911 in S. ionosma (26.67%). and 1711 + 41 in V. lamellata (31.88%). In general, there were relatively high frequencies of cells with normal chromosome behavior at all stages of meiosis. V. lamellata had the highest frequency of normal chromosome behavior at Telophase 11 (98.0%). Laggards and non-congression of chromosomes were also present but only at low frequencies. V. lamellata had the lowest frequency of cells with laggards at Telophase II (2.0%) while P. schilleriana had the lowest percent cells with non-congression at Metaphase 1 (2.78%). High percentages of pollen fertility were obtained because of high frequencies of normal chromosome behavior at meiosis — 89.61% in P. philippinensis, 93.53% in P. schilleriana, 84.67% in S. ionosma, and 92.60% in I'. lamellata.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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