Mitotic effect of Ginger Lily (Hedychium coronarium Koenig) extract on onion (Allium cepa L.)

Date

3-2000

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

The mitotic effect of Hedychium ooronarium Koenig (ginger lily) extract on .4/hum cepa L (onion root cells) was evaluated using the acetocarmine squash technique Water and chloroform rhizome extracts were prepared (approximately 20%w/v) and diluted to 25, Su, and 75% stock solution Approximately one centimeter onion root tips were treated with the solution for 6, 12 and 24 hours ('ells treated with water and chloroform ginger lily extracts showed a significant macaw in mitotic indices at a 0.05 (P-valuev0 010* and 0 044*) irrespective of concentration and length of exposure periods However, significant differences on mitotic indices were obtained in different concentration of the water extract (P-value 0.014*) and various exposure periods in the chloroform extract (P-value 0 040*) of ginger lily extract Mitotic indices was highest in root tip cell treated for 12 hours at 50% ginger lily water extract (mean - 0 5632) and in those treated for 24 hours at 25% ginger lily chloroform extract (mean = 0 6088) Significant interaction of the concentration and exposure periods was obtained in the frequency of prophases (P-value = 0 049*). Accumulation of prophase was remarkable in all treatments showing a decreasing trend of mitotic phases as mitosis progresses Very few abnormalities were observed indicating no significant mutagenic effect of the ginger lily rhizome extracts

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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