Comparative isozyme analysis of glucose-6-phosphate dehydrogenase and esterase of the giant honeybee (Apis dorsata Fab.) at different developmental stages

Date

3-1998

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

Esterase showed the existence of three presumptive loci: Est-1, Est-2 and Est-3. Est-1 was expressed in all developmental stages In larva, nurse bee and adult, Est-1 was observed to have three presumptive alleles: S (slow), M (moderate) and F (fast) In pupa, Est-1 showed only two alleles: S and M. Est-2 and Est-3 were expressed only in pupa and adult In pupa, Est-2 and Est-3 were polymorphic with two presumptive alleles: S and F. However, in the adult Est-2 and Est-3 were monomorphic with only the allele F observed. Glucose-6-phosphate dehydrogenase showed the existence of two presumptive loci. G-6-Pd-1 and G-6-Pd-2 G-6-Pd- I was expressed and found to be monomorphic in all developmental stages. It showed only the presumptive allele S On the other hand, G-6-Pd-2 was polymorphic in all developmental stages In larva and nurse bee, G-6-Pd-2 showed three presumptive alleles: S, M and F. In pupa and adult, only the presumptive allele S and M were observed The genetic similarity values obtained based on the Jaccard similarity index showed that there is a high variability in the electrophoretic band patterns of glucose-6-phosphate dehydrogenase and esterase in Apis dorsata Fab.at different developmental stages. This suggests that there are genes like Est-2 and Est-3 which are activated at pupa and adult stages but switched off at larva and nurse bee stages causing the expression of different phenotypes

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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