"Effects of flavonoids on pectate lyase and protease activity of Pseudomonas solanacearum E.F Smith"

Date

4-1995

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

Adviser/Committee Chair

Evelyn Mae T. Mendoza

Abstract

Biochemical interaction between bacterial enzymes and flavonoids were analyzed in terms of changes in pectate lyase and protease activity upon treatments with flavonoids. Activity of the 2 enzymes inside the bacterial cells were also determined. For pectate lyase, significant inhibition of activity by all flavonoids tested was observed. Catechin, 66 % maximum inhibition at 25 uM; chrysin, 78 % inhibition at 100 uM; apigenin, 70 X at 10 uM; rutin, 49 X at 10 uM; quercitin, 58 X at 6 uM and naringin, 66 % at 25 uM. IC50 values for catechin, chrysin, apigenin, quercitin and naringin were 9.15, 9.6, 7.11, 1.8, and 4.40 uM respectively. Rutin was not able to give 50 % inhibition. Generally, treatments with flavonoids increased protease activity as compared with the control. Fifty uM quercitin resulted in maximum increase of activity of 236 X. At 100 um concentrations of catechin, naringin,

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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