Genetic variability in salinity tolerance at tolerance at seedling, vegetative and reproductive stages of rice (Oryza sativa L.)

Date

4-2004

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Co-adviser

Glenn B Gregorio

Abstract

Ten rice (Oryza sativa L.) lines with known tolerance for salinity were grown in both saline and normal culture solutions under controlled conditions at EC12 dS/m during the seedling and vegetative stages and EC6 dS/m during the reproductive stage. Differences in response to salinity stress were noted in all growth stages. Some lines responded differently, depending on the growth stage where salinity stress was introduced. Visual scoring of salt damage and plant height were assessed during the seedling stage. Significant differences among the lines were observed. At the vegetative stage, shoot Na and K concentrations were measured, along with other agronomic traits. Yield components were measured at the reproductive stage. IR29 was susceptible while 1R63295-AC209-7 was tolerant at all growth stages. IR66946-3R-78-1-1's tolerance decreased as it matured. Genetic variability or the phylogenetic relationship between lines was also determined by employing 134 molecular markers (microsatellite-SSR). The 10 lines segregated into three clusters with one line IR65192 diverging from the rest. Clusters that were generated were associated with the parental lineage of the genotype. Polymorphism was generated by 94 Rice Microsatellite markers. The PIC (Polymorphism Information Content) values ranged from 0.180 to 0.790 with a mean of 0.510.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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