"Genetic diversity in seed proteins of ''Kondol'' Benincasa hispida (Thunberg ex murray ) cogniax using SDS -page"

Date

10-1997

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Co-adviser

Abella C. Dela Vina

Abstract

The genetic diversity in total seed protein among 32 accessions of "kondol" Benincasa hispida (Thunberg ex Murray) Cogniax or wax gourd was determined using SDS-PAGE. A total of 18 major protein bands were resolved with bands 11 and 14 as more intense. Bands 11 and 14 are common to all accessions while bands 6 and 8 are unique to accessions 18 and 26 respectively. There was high polymorphism observed with 12 banding pattern (BP) among 32 accessions. These are BP1 (band 6, 11, 14 and 15), BP2 (bands 1, 2, 5, 7, 9, 11 and 14), BP3 (BP2 plus band 3), BP4 (BP2 plus band 17), BP5 (bands 7, 9, 11, 13, 14, 15, 16, 17 and 18), BP6 (8P3 plus bands 17 and 18), BP7 (BP5 plus band 8), BP8 (hands 2, 3, 4, 5, 7, 9, 10, 11, 14, 15, 17 and 18) BP9 (BP6 plus bands 4, 10, and 15), BP10 (bands, 1, 2, 5, 7, 9, 10, 11, 12, 14, 15, 16, 17, and 18), BP11 (BP6 plus bands 10, 12 and 15), and BP12 (bands 2, 3, 5, 7, 9, 10, 11, 12, 14, 15, 16, 17 and 18). Band pattern 6 is the most common BP occurring at 28125%, BP1, BP5, BP7, BP8, BP9, BP10, BP12 were found to be unique to accessions 18, 28, 26, 11, 31, 33 and 22, respectively. Highest similarity can be found between BP2, BP3 and 8P4 (87.5%) while BP1 and BP6 has the least similarity (16.6%). Among the 32 accessions the highest similarity index is 100% having the same band pattern. While the lowest similarity of 16.6% can be found between accession 18 and 14, 19, 29, 35, 5324, 5393, 5652, 5861 and 8160. This differences is caused by presence of cultivar groups and low popularity in cultivation which led to the preservation of genetic diversity. Due to limited sampling, the genetic diversity cannot conclusively be associated with geographic distribution.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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