Preparation and partial characterization of crude monoglycerides from lipase catalyzed synthesis

Joan T. Acay

Date

3-1999

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Marivic S. Lacsamana

Abstract

Enzymatic glycerolysis of coconut oil for the production of monoglycerides was carried out using a lipase from Rhizopus delemar. The highest amount of 75.12% monoglycerides was obtained by incubating the reaction mixture for 24 hours at 40 C with 1.1 (v/v) glycerol-oil ratio and 1.0 gram lipase in 10.0 mL solution. Among the parameters investigated, only the amount of lipase showed no marked effect on the amount of monoglycerides produced. Identification and quantification of the products were done using Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC), respectively. A mixture of monoglycerides, diglycerides, starting triglycerides and free fatty acids were detected. The monoglycerides formed were isolated then esterified and the fatty acid component was determined using Gas Chromatography (GC). The fatty acids namely caproic, lauric, myristic, palmitic and stearic acid were identified. The mixture of monoglycerides was found preferentially soluble in chloroform.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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