Isolation, purification and partial characterization of bioactive compounds from Bayati (Anamirta cocculus Linn.) seeds and evaluation of their toxicity against larva of dengue mosquito (Aedes aegypti L.) (Diptera: Culicidae) / Hazzel Joy Licoto Adra ; Annabelle T. Abrera, adviser.

Date

12-2014

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Annabelle T. Abrera

Abstract

Preliminary phytochemical screening on bayati (Anamtria cocculus Linn.) seeds, showed presence of alkaloid, saponin and terpenoid. Survey of the larvicidal activity of bayati seeds against At-des aegypti larvae showed a percent corrected mortality of 100.00 ± 0.00, 80.83 ± 5.20 and 2.50 ± 2.50 for 5000 ppm, 2500 ppm and 1000 ppm at 48 HAT, respectively. Selection of the most suitable solvent showed that ethanol has the highest percentage yield of extracted compound (5.63 g). All crude extracts showed a 100 % larval mortality at 24 and 48 HAT which indicates that all solvents can extract the bioactive compounds of bayati seeds. The bioactive components of seeds (500g) were extracted with ethanol. Mortality was observed at 400 ppm (27.50 ± 2.50 %) and 800 ppm (31.67 ± 3.82%) at 48 HAT. Fractionation of the crude ethanolic extract through silica gel 060 column chromatography using hexane, hexane-ethyl acetate (1:1), ethyl acetate, ethyl acetate-methanol (1:1) and methanol as eluting solvents yielded eight fractions. Fractions, 2, 3 and 4 formed crystals during incubation at 4°C. Evaluation of the three fractions revealed that fraction 2 is the most bioactive (% mortality = 16.67 ± 5.77 % for 160 ppm at 48 HAT). IR analysis of fraction 2 (MP: Trial 1 — 66-67 °C, Trial 2 = 67 °C) and fraction 3 (MP: Trial 1 = 210-218 'V, Trial 2 = 210-214 °C) suggests presence of alcohol, ketone, ether and ester. Additional peaks were found in fraction 3 which indicate presence of aliphatic group and alkene. H NMR further suggests that the possible identity of fraction 3 is picrotoxinin.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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