Quantitative determination of glycoalkaloid content among transgenic and non-transgenic eggplants (Solanum melangena)

Date

4-2009

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Myrna S. Rodriguez

Abstract

A method for the determination of total glycoalkaloid content in cloned and commercial cultivars of eggplants was assessed for validity. The glycoalkaloid in eggplants were extracted with acetic acid and was precipitated with concentrated ammonium hydroxide. The precipitate was dissolved in methanol titrated with 0.067% bromphenol blue, 10% phenol in absolute methanol. The standard curve obtained was linear in the range of 50-1000ppm of a-chaconine. It was found out that Mara and DLP Bt+ samples had lower amount of TGA while the Mara and Dagupan Long Purple (DLP) Bt- had higher amount of TGA compared with its non-transgenic mother plant. Mara eggplants had higher TGA content than DLP eggplants. Those eggplants obtained from several wet markets in Laguna had higher amounts of TGA than those cloned eggplants. TGA content was expressed in g/100g fwt. With the spiking experiments, the calculated percentage recovery values were 73.68% and 80.58% for the additional 500ppm and 1000ppm of a-chaconine standard solution. There were significant differences (P < 0.05) in total glycoalkaloid content between the cloned and wet market samples. However, no significant differences were obtained between the control and the treatments for each cloned eggplants.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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