Preliminary analysis of flavonoids in propolis of honey bee (Apis mellifera Linnaeus) and stingless bee (Trigona briroi Friese) from the Philippines and honey bee (Apis mellifera Linnaeus) from Canada

Date

5-2011

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Jose Rene L Micor

Co-adviser

Cleofas R Cervancia

Abstract

Flavonoids are considered as key compounds responsible for the biological activities of propolis. In this study, the flavonoid compounds present in different propolis samples were investigated. LC-MS was employed to analyze the flavonoid compounds present in the propolis extracts. Apis mellyira and Trigona biroi propolis extracts from the Philippines are characterized by the presence of flavonoids such as pinobanksin-5,7- dimethyl ether and pinobanksin-3-0-(butyrate or isobutyratc). Pinobanksin-3-0- pentenoate and pinobanksin-3-0-hexanoate were also present in Apis mellifera propolis (Philippines) while apigenin, pinocembrin-5-methyl ether and kaempfcride were identified in Trigona biroi propolis (Philippines). Apis mellifera propolis from Canada is characterized by a number of flavonoid compounds which are not identified on the native propolis samples such as pinobanksin and its derivatives, pinocembrin, chrysin, chrysin-S-methyl ether and quercetin. The flavonoid content (flavone and flavonol) of the extracts was determined colorimetrically using AICI3 with quercetin as standard. Apis mellifera propolis extract from the Philippines gave the lowest flavonoid content (6.44 ± 1.46 mg quercetin equivalent (QE)/100 g extract) followed by Trigona biroi propolis extract also from the Philippines (9.40 ± 0.47 mg QE/100 g extract). Apis mellifera propolis extract from Canada gave the highest flavonoid content at 36.00 ± 8.07 mg QE/100 g extract.

Language

Filipino

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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