Isolation, characterization and bioassay of luyang dilaw (Curcuma longa Linn.) components against cabbage moth (Crocidolomia binotalis Zeller)

Date

4-2003

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Hidelisa P. Hernandez

Co-adviser

Belen Morallo-De Jesus

Committee Member

Veronica Sabularse

Abstract

Dried, ground rhizomes of luyang dilaw (Curcuma long(' Linn) were successively extracted with petroleum ether and dichloromethane The crude petroleum extract was an orange oily mixture while the crude dichloromethane extract was a red orange paste. The petroleum ether extract was found to have ovicidal toxicity against cabbage moth while the dichloromethane extract was found to have antifeedant activity against 2' and 3' instar larvae of cabbage moth Rapid column chromatography of the dichloromethane extract yielded four fractions. Fraction 1 is a light yellow oil, while Fraction 2 is an orange paste. Fractions 3 and 4 are orange solids. Fraction 1 exhibited the highest toxicity followed by Fraction 2, but both were phytotoxic. Fractions 3 and 4 have high antifeedant activity against 2' instar cabbage moth larvae. Fraction I contains one major component, which is a colorless UV absorbing oil, Component 1. Fraction 2 contains a colorless non-UV absorbing oil, Component 2; yellow oils, Component 3 and 4. Fraction 3 contains orange solid, Components 5 and 6. Fraction 4 contains also contains Components 5, 6, and an orange solid, Component 7. Mass spectrometry and infrared spectroscopic analysis suggest that Component 1 is an or/ha-substituted aromatic ring with alkene functional group. Infrared analysis suggests that Component 2 has a ketone group conjugated with an aromatic ring and has an alkene; Component 3 has an aromatic and alkene functional groups, and Component 4 contains an aromatic ring, ketone and alkene functional groups. The melting point of Components 5, 6 and 7 are 60-65°C, 55-60°C and 205-210°C, respectively. Infrared analysis of Component 5 indicated an aromatic ring, while both Components 6 and 7 possess aromatic ring, ketone and alkene functional groups.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2003 A13 G66

Document Type

Thesis

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