Enzymatic preparation and partial characterization of isoamyl acetate in solvent-free system

Date

3-1999

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Co-adviser

Ruby A. Ynalvez

Committee Member

Macario P Catahan

Abstract

Freeze-dried lipase front Rhizopus delemar with specific activity of 27.43 U/mg was used to catalyze the synthesis of isoamyl acetate in a solvent-free system. Maximum amount of isoamyl acetate in the final reaction mixture was 90.47% (v/v) based on gas chromatography; this yield of ester was obtained using 2.0M isoamyl alcohol, 0.51A1 acetic acid and 0.25g lipase incubated for 6 hours with shaking at 30°C Identification, quantitation and partial characterization of the product were done using gas liquid chromatography. ammonolysis, ferric hydroxamate test and solubility tests, respectively

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1999 C4 G85

Document Type

Thesis

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