Development of rapid technique for the detection of chlorinated insecticide residues

Date

10-1997

Degree

Bachelor of Science in Agricultural Chemistry

Adviser/Committee Chair

Amelia W. Tejada

Co-adviser

Nieva L. Basilio

Committee Member

Veronica P Migo

Abstract

Rapid technique for the detection of chlorinated insecticides was developed using micro thin layer chromatography Response of chlorinated insecticides to various kinds of adsorbents , solvent systems and chromogenic reagents was investigated. Among the different TLC systems tested, precoated chromatosheets , DC Plastikfolien Silica 60 F254 and n-hexane-acetone ( 60.40 ) as developing solvent gave superior results. The minimum detect-able limit ranges from 0.2-0.9 ug for DDT, its analogues and aldrin, 2.5-3.0 ug for the isomers of endosulfan, 5.0-7.0 ug for dieldrin, heptachlor and chlordane. The validity of the developed method was tested in spiked vegetable samples by gas chromatography and gave recoveries ranging from 86 - 98 %. The developed technique was packaged into a rapid field kit. This consist of the precoated plates, extracting solvent, vial for extraction, developing solvent, developing chamber, capillary tube, forceps, acetone, UV flashlight

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1997 A13 H47

Document Type

Thesis

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