Isolation of ipil-ipil (Leucaena leucocephala (Lam.) de Wit.) seed protein

Date

3-1979

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Lilia S. P. Madamba

Abstract

The composition of seeds of two varieties of ipil-ipil, Sta. Elena and K-23 was determined. Proximate analysis revealed a high crude protein content (22.15% and 25.13%, IFS for Sta. Elena and K-23, respectively). Mimosine was found to be high in both varieties (9.21:4 and 8.59%. MFB). The effect of pH on the percentage nitrogen extract-ability showed that the amount of nitrogen extracted at alkaline pH was greater than that produced at either neutral or acidic pH. The minimum dispersion point or isoelectric point for Sta. Elena was at pH G.0 and for K-28 at pH 4.0. The best conditions for protein isolation was found to be extraction at pH 11.0 and precipitation at pH 4.0. The percentage protein of the protein isolate was 70.31% and 69.00% for Sta. Elena and K-28, respectively. The bulk of mimosine accumulated in the supernate (83-88%) with negligible amounts in the protein isolate (less than 2:0. Of the total seed protein, the percentage isolated at isolation pH 11-4 was 8G.5G and G6.52% for Sta. Elena and K-28, respectively.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1979 A13 H53

Document Type

Thesis

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