Isolation and molecular weight determination of Globulin Subunits from coconut (Cocos nucifera Lin.) residue sapal

Date

10-1995

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Co-adviser

Florinia E. Merca

Abstract

Globulin storage protein of coconut residue sapal was isolated using 25 mM borate buffer (pH 8.6) containing 10% NaCI. Gel chromatography on Sephadex 0-100 exhibited a single peak with an estimated molecular weight of 150 ± 6 Ed. Electrophoretic property of the globulin under denaturing condition revealed two bands with relative mobilities of 0.84 and 0.93. The molecular weights of the bands were found to be 16,600 and 13,800 daltons, respectively. Solubility at different pHs (pH 1 to pH 12) gave values greater than 60% solubility except at its isoelectric pH where solubility dropped to about 1% (1.0% at pH 5 and 0.9% at pH 6).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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