Application of pectinase on the extraction of essential oils from rose (Rosa Spp.) petals

Date

4-2007

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita M. Espino

Co-adviser

Florinia B. Merca

Committee Member

Dolores P. Buquiran

Abstract

Extraction of essential oil from rose (Rosa Spp.) petals was conducted using the pectinase. Petroleum ether was used as the extracting solvent for the recovery of essential oil. Several parameters were optimized to maximize essential oil yield. These parameters included enzyme concentration, incubation time and sample buffer dilution ratio. The optimized conditions obtained were as follows. enzyme concentration = 0.5%, incubation time = 16 hours, sample:buffer dilution ratio = I (w:v). Using the optimized conditions, an essential oil yield of 0.160% (grams essential oil per 100 grams fresh sample) was obtained for the enzyme treated and 0 088% oil yield for the control (without enzyme) This represented a 1.82 fold increase in essential oil yield Physical and chemical analyses showed that the enzyme treated rose petals had better quality than that of the untreated sample. An acid value of 5.19 was determined for the untreated sample while 3.55 for the enzyme treated. For the specific gravity, 0.748 and 0.739 were obtained for the untreated and enzyme treated samples respectively

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2007 C4 J37

Document Type

Thesis

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