Synthesis and determination of antimicrobial activity of 1- monolaurin and coconut 1- monoacylglycerol mixture

Date

3-2002

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn B. Rodriguez

Co-adviser

Maxima E. Flavier

Committee Member

Evamarie P. Capareda

Abstract

1-Monolaurin was synthesized from lauric acid and glycerol. Protection of the 1,2-hydroxyl groups of glycerol was achieved by conversion to isopropylidene glycerol. Transesterification of methyl dodecanoate to the free primary hydroxyl group was then carried out using potassium carbonate as catalyst. Removal of the protecting group by cold acid hydrolysis afforded the 1-monolaurin with 89% yield. Coconut 1-monoacylglycerol mixture was synthesized from coconut oil using the same method employed for 1-monolaurin. A 92% yield by weight of the acylated dioxolane was achieved. 1-Monolaurin, coconut 1-monoacylglyccrol mixture, and lauric acid were evaluated in Nutrient and Potato Dextrose Agar for antimicrobial activity against Escherichia cob (gram - bacteria), Staphylococcus aureus (gram + bacteria), Bacillus cereus (gram -f, spore former) and Saccharomyces cerevisiae (yeast, spore former) at ambient temperature using the modified filter paper disc agar diffusion method. I. Monolaurin proved to active against B. aureus up to 10 ppm while it inactivated S. aureus and S. cerevisiae at 5 ppm. Coconut 1-monoacylglycerol mixture showed a consistent minimum antimicrobial concentration to B. cereus, S. aureus, and S. cerevisiae at 5 ppm. Laurie acid was able to inhibit the growths of S. aureus at 50 ppm, but only inhibited B. cereus, and S. cerevisiae at 100 ppm, respectively. All the three compounds, however, were not active against K. cols. up to 1000 ppm. The results showed that 1-monolaurm, coconut 1-monoacylglycerol mixture, and lauric acid only inhibited the yeast and the grain positive bacteria. Coconut 1- monoacylglycerol mixture was as effective as 1-monolaurin in (wrens, and S. cerevisiae but showed better effectivity against B. cereus, an endospore forming bacteria.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2002 A13 L33

Document Type

Thesis

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