Partial purification of a lectin from Jatropha curcas Linn. seeds by isoelectric precipitation and its effect on selected plant pathogens

Date

4-2011

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Marivic S. Lacsamana

Co-adviser

Marina P. Natural

Committee Member

Veronica C. Sabularse

Abstract

A non-blood type specific lectin was isolated from the seeds of Jatropha curcas seeds by extraction with 5 mM phosphate buffer containing 0.2 M NaCI at pH 7.2. Further purification was attained by 60% ammonium sulfate fractionation followed by isoelectric precipitation. Isoelectric precipitation of the redissolved precipitate obtained at 60% saturation with (N1-14)2SO4 at pH 8.54 revealed a single-band in native-PAGE suggesting the possible purity of the lectin isolated. However. SDS-PAGE revealed three bands suggesting that isoelectric precipitation resulted in the formation of protein aggregates containing the lectin of interest and other proteins resulting in partial purification of the lectin. Estimated molecular weights of the three proteins were 29.06 KD, 20.92 KD and 16.54 KD. The protein with a molecular weight of 29.06 KD may be curcin. Filter paper disc assay of the partially purified lectin at pH 8.54 using known plant pathogens such as Ralstonia solanacearum. Pectobacteriurn carotovorum. Xanthomonas orreae pv. or)7.ae, Rhizoctonia solani and Bipolar& maydis gave negative results.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2011 A13 L37

Document Type

Thesis

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