Preparation and evaluation of in-house reference materials for trace metal analysis using rice grain (Oryza sativa Linn.) and saluyot leaves (Corchorus olitorius Lam.)

Date

4-2005

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Maxima E. Flavier

Co-adviser

Evelyn B. Rodriguez

Committee Member

Teofila D. C. Villar

Abstract

In-house reference materials were prepared from powdered rice grain and saluyot leaves for use in trace metal analysis. The samples were spiked with known amounts of cadmium, copper, lead and zinc. The samples were mixed and homogenized thoroughly to make sure that there is uniform distribution of the four metals (cadmium, copper, lead and zinc) in the samples. The prepared materials were subjected to two methods of oxidizing organic matter, the wet digestion and dry ashing methods. The two methods of digestion were compared in terms of their precision and accuracy with respect to the analysis of cadmium, copper, lead and zinc. The wet digestion its method was found to have higher precision in terms of repeatability and reproducibility as compared to the dry ashing method. The accuracy of the methods varied significantly considering the metal and the sample under study. Statistical comparison of the methods for copper and zinc using DMRT ( a= 0.05) revealed t hat dry ashing yielded higher m can p creche recoveries for both rice and saluyot. The homogeneity of the prepared in-house reference materials were evaluated by dividing each sample into six subsamples and determining the concentration of cadmium,

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2005 C4 L37

Document Type

Thesis

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