Preparation and partial characterization of diglycerides from lipase - catalyzed reaction

Date

10-1999

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Co-adviser

Marivic S. Lacsamana

Committee Member

Macario P. Catahan

Abstract

A crude lipase preparation from Rhizopus delemar, with a protein content of 6.40mg/ mL and specific activity of 6.03U/ mg protein was used to catalyze the production of diglyceride or diacylglycerol (DAG) using coconut oil as substrate. Mixture of triglycerides, diglycerides, monoglycerides and free fatty acids were detected using Thin Layer Chromatography (TLC). The tri-, di-, and monoglyceride contents were measured through High Performance Chromatography (HPLC). The effects of alcohol chain length, temperature, incubation time, amount of lipase and coconut oil- alcohol ratio on diglyceride production were investigated. The highest amount of 52.06% DAG was obtained by incubating the reaction mixture consisting of coconut oil- ethanol at a ratio of 1:4 (mole/mole) and 1.0g lipase for 18 hours at 45°C. The diglyceride formed was isolated then esterified and the fatty acid composition was determined by Gas Chromatography (GC). The DAG was composed of 3.72% capric acid, 36.12% lauric acid. 10.20% myristic acid and 5.18% palmitic acid. The diglyceride was found to be soluble in slightly polar solvents like propanol, chloroform and diethyl ether.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

"LG 993.5 1999 C4 /L38"

Document Type

Thesis

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