Biological activities of a lectin purified from the leaves of Schefflera odorata (Blanco) Merr. & Rolfe

Date

10-2004

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Marivic S. Lacsamana

Abstract

A lectin was isolated from the leaves of Schefflera odorata (Blanco) Merr. & Rolfe by extraction with 0.02 M phosphate buffered saline (pH 7.2). The lectin was purified by ammonium sulfate precipitation and gel filtration chromatography on Sephadex G - 150. The lectin was non-blood type specific since it agglutinated the erythrocytes from human blood types 0, AB, B and A. The homogeneity of the lectin was confirmed by polyacrylamide gel electrophoresis (PAGE) under non-denaturing condition which revealed a single band. SDS — PAGE analysis showed that the lectin is monomeric with an estimated molecular weight of 225 kD. The purified lectin did not inhibit the growth of pathogenic plant microorganisms such as Peciobacterium carotovorum, RaIstonia solanacearum, Diplodia macrospora and Rhizoctonia solani , and had no toxic effects on corn borer, Oarinia furnacalis neonates. However. it was able to inhibit the growth of Xanthomonas oryzae pv. oryzae and caused the inhibition of mycelial growth of Fusarium sp. at a concentration of 100 ppm.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2004 L48

Document Type

Thesis

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