Immobilization of lipase from haustorium of germinating coconut (Cocos nucifera) by adsorption method

Date

3-1995

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Abstract

A lipase from haustorium of germinating coconut (Cocos nucifera) with a protein content of 0.14 mg/ml was immobilized by adsorption method using silica gel. Immobilization shifted the optimum pH and temperature of the lipase from 6.0 to 5.0 and from 350C to 45°C, respectively. Determination of the effect of storage time on the activity of free and immobilized lipase showed a gradual decrease in the activity of immobilized lipase while free lipase activity drastically changed. Gas chromatographic analysis of the products of the lipolytic action of the free and immobilized lipase on coconut oil showed that almost all of the fatty acids present in coconut oil namely caproic acid (Cal, caprylic acid (Cal, cap!, acid (C10), lauric acid (C12), rnyristic acid (C14 and palmitic acid (C16) were hydrolyzed by both free and immobilized lipase.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1995 C4 L66

Document Type

Thesis

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