Isolation, identification and structure elucidation of the major components of the essential oil of river red gum (Eucalyptus camaldulensis Denhn) leaves

Date

6-2004

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn B. Rodriguez

Co-adviser

Evamarie P Capareda

Committee Member

Maxima E. Flavier

Abstract

Hydrodistillation was employed in the extraction of the essential oil of river red gum (Eucalyptus camaldulensis Denhn) leaves. The oil obtained is clear, yellowish and viscous liquid. This method gave an average % yield of 0.35 % (w/w). The oil W. subjected to column chromatography (silica gel 60) by gradient elution. TLC analysis showed that there were four major groups namely Ft. F2, F3 and F4. Only 1-72 gave one spot in the TLC. F4 was purified further using the chromatotron to yield four group fractions namely A, B. C and D, all with one spot in TLC. GC-FID analysis showed that F2. A and B had only one peak. F2, A and B. found to be pure by TLC and GC, were subjected to proton ('H) NMR spectroscopic analysis. The spectra of F2 and A were found to be identical while that of B was slightly different. F2 and B were further subjected to carbon (13C) NMR (Broadband. DEPT 135 and DEPT 90) spectroscopic analysis. 11-1 and '3C NMR analysis showed that F2. the major component, is the known compound 1, 8-cineole. while B, a minor component, is a sesquiterpene. Thus, the essential oil from the mature leaves of E camaldulensis belongs to chemotype 1, containing 82 % I. 8-cineole and low sesquiterpenes.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2004 C4 L83

Document Type

Thesis

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