Survey of lectin activity in the seeds and the leaves of selected leguminous plant species

Date

3-1988

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Florinia E. Merca

Abstract

Seed and leaf - protein extracts of eight leguminous plant species namely: Acacia senegal (common name: Gum acacia, Eng.), Caesalpinia coriatCa L. (en: Divi-divi, Eng.), C. pulcherrima L. (Cn: Kaballero), Crotolaria retusa L., Desmodium purpureum (Cn: Di)sit-dikit, Tag.), Mimosa pudica L. (Cn: Makahiya, Tag.), moringa oleifera Las. (Cn: Salungay, Tag.), and Pachyrrizus erosus L. (Cn: Singkamas, Tag.; Turnips, Eng.), were surveyed for lectin activity using hemagglutination assay with a panel of erythrocytes (from human blood types A, B, 0 and AB and carabao and swine blood). Only the leaf protein extracts of Leucaena leucocephala (Lnk) De Witt (Cn: Ipil-ipi(, Tag.) and Tamarindus indica L. (cn: Sampalok, Tag.) were assayed since their seed extracts were previously stu-died. Extraction of the seed and leaf proteins were done with phosphate buffered saline (p)1 7.2) and partial purification of the extracts using ammonium sulfate fractionation. Trypsination of the erythrocytes was made to determine its effects on aggluti-nation. The seed and leaf extracts of Caesalpinia coriarea, Acacia r.enegal, rlesmodium purpureum and Mimosa pudica exhibited a gene-rally positive agglutination response to all types of human blood

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1988 C4 L83

Document Type

Thesis

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