Hydrolysis of potato starch using glucoamylase immobilized on diethylaminoethyl-sephadex

Luz Z. Lucas

Date

6-1979

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ernesto J Del Rosario

Abstract

Potato starch was hydrolyzed into sugar using glucoamylase immobilized on diethylominoethyl (DEAE)-Sephadex. The activity of the immobilized enzyme varied with the flow rate ,starch concentration, and acid pretreatment of the substrate. Using the some starch concentration, foster flow rates cause,: conversion of starch and produced low sugar concentration in t e eluate. On the other hand, using the same flow rate, low starch concentration produced higher per-cent conversion of starch but produced lower sugar concentration than high starch concentration. Higher conversion values and greater sugar concentrations were obtained when tie substrate was _pretreated with acid.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1979 C4 L83

Document Type

Thesis

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