Enzymatic synthesis of hydroxamic fatty acids from coconut oil by lipase from Carica papaya latex

Date

4-2005

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Co-adviser

Dolores P. Baquiran

Abstract

Crude lipase from Caries papaya latex was used to catalyze the enzymatic synthesis of hydroxamic fatty acid (FIFA) using coconut oil, lauric acid, and methyl laurate as substrates. The effects of amount of hydroxylamine-hydrochloride (NH201-1- HCI), incubation time incubation temperature and amount of enzyme were studied and optimized. The HFA formed were analyzed using Gas-Liquid Chromatography (GLC). Percent laurylhydroxamic acid obtained were 90.67%. 87.81%, and 69.20% for methyl laurate, lauric acid and coconut oil, respectively. The FIFA was purified and purity was determined by IR spectroscopy.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2005 C4 M34

Document Type

Thesis

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