Histochemical study of adenosine triphosphatase in the solid endosperm of coconut

Date

11-1968

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Antonio D. Bustrillos

Abstract

The presence, distribution and some physico-chemical properties of adenosinetriphosphatase (ATPase) were demonstrated histochemically in the solid endosperm of coconut. The histochemical reaction demonstrated by the production of brown spots (PbS precipitate) indicated the sites of the enzyme activity in the tissue. The study showed that the hydrolysis of ATP was optimum at pH 5.0-5.6 and A.0-9.2, the precipitate production in the alkaline range being probably due to alkaline phosphatase which also hydrolyzes ATP. Thirty to sixty minutes of incubation at 37°C was sufficient to have a maximum production of precipitate. Five milligrams of ATP per 36 milliliters of the medium was enough substrate for the formation of the enzyme-substrate complex. A decreasing gradient of ATPase concentration was observed from endosperm tissues near the location of the "eyes" (portion I) to the tissues near the bottom (portion III) of the coconut. The same trend was also observed from the tissues near water layer to the tissues near the testa.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1968 A13 M34

Document Type

Thesis

This document is currently not available here.

Share

COinS