Isolation and structure elucidation of biologically active non-protein fractions from Synapta maculata (Chamisso and Eyesenhardt)

Date

4-2007

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Florinia E. Merca

Co-adviser

Dolores P Buquiran

Committee Member

Josefina L. Solivas

Abstract

Crude extracts from Synapta maculates were obtained through sequential extraction with ethanol, ethyl acetate, and n-hexane. The crude extracts were assayed for cytotoxicity using the brine shrimp (Anemia saliva) lethality assay (BSLT). The ethyl acetate extract (EA) was found to exhibit the highest biological activity among the crude extracts at 500 ppm. Flash column chromatography of the ethyl acetate extract yielded five fractions (EA] — EAS). EA2, being the most active fraction, was further fractionated to obtain three fractions (EA21-EA23). EA21 was found to be the most active fraction against A. sedum at 500 ppm. LCso for EA21 using BSLT was found to be 557 ppm. IR analysis of EA21 indicated the presence of a carboxylic acid and an alkene in its component(s). GC-MS analysis of EA21 shows three major peaks in the chromatogram (EA211-EA213). From the mass spectral libraries and from the fragmentation patterns, EA211 is probably squalene while EA2 12 and EA213 are probably hexadecanoic acid and octadecanoic acid, respectively.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2007 C4 M34

Document Type

Thesis

This document is currently not available here.

Share

COinS