Comparison of phenolic compounds from exudates of plants used by stingless bees (Trigona spp.) as propolis sources

Date

5-2011

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Jose Rene L. Micor

Co-adviser

Cleofas R. Cervancia

Abstract

Phenolic compounds from five plants that are utilized by stingless bees as propolis source namely mango (Mangifera indica L.). jackfruit (Ariocarpus heterophyllus Lam), avocado (Persea americyma Mill), rambutan (Nepheliatn lappaceurn L.) and pill (Canarium ovatum Engl) were extracted from tree exudates and trunks using acetone. Liquid-liquid extraction was then applied using diethyl ether and pet ether. The total phenolic content of the ether extracts was then determined using the Folin-Ciocalteau assay. Mangifera indica was found to have the most phenolic content of all the extracts with 94.44 ntg gallic acid equivalent followed closely by C. ovaturn with 91.98 while P. americana registered the lowest amount of phenolic compounds. A small aliquot of each ether extract was then subjected to an EIPLC-DAD-MS analysis to characterize the possible compounds present in the extracts which are also found in propolis samples. Canarium ovanon registered the most number of distinct peaks in the negative and positive ion chromatogram as well as the most number of possible compounds characterized. Artepillin C was found to be possibly present in all the ether extract while certain phenolic compounds appeared only in a single extract. Quercetin and methoxy-pinobanksin only showed in the chromatogram of AL indica while p-coumaric-butenyl ester and dicaffeoyl quinic acid appeared only in P. americana chromatogram. The results were also compared to the study of Lechuga (2011) on the possible phenolic compounds isolated from the propolis of T. hiroi, the stingless bee that is native in the Philippines, and it showed that there are three phenolic compounds from its propolis that is also present in plant exudate extracts. These compounds are namely (1) Artepillin C, (2) luteolin-5-methyl-ether and (3) pinobanksin-5,7-dimethylether. From the results obtained in this study all of these three phenolic compounds were isolated from N. lappaceum and C ()vellum extracts, which we can say for now are the major contributors of propolis amongst the other exudates studied. The other plants have only two out of these three compounds.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2011 C4 M38

Document Type

Thesis

This document is currently not available here.

Share

COinS