Extraction of proteolytic enzymes from tiger prawn (Penaeus monodon) hepatopancreas

Gemma C. Mose

Date

4-1993

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Veronica C. Sabularse

Abstract

Approximately 90 g of hepatopancreas were removed from 1 kg of mixed sexes of Tiger Prawn, Penaeus monocion, and crude proteolytic enzymes were extracted from the hepatopancreas.

The crude enzyme preparation from the prawn had an enzyme activity of 2.33 units per mL and a specific activity of 0.142 units per mg protein. This also produced a solution of 16.46 mg protein per mL and the yield was 17.45 mg protein per g hepatopancreas.

The ammonium sulfate precipitation at 0 to 35% fractionation and at 60 to 75% fractionation increased the specific activity of the crude enzyme extract. These results suggest the probable presence of at least two proteolytic enzymes in the crude enzyme extract.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1993 C4 M67

Document Type

Thesis

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