Isolation and characterization of allelochemicals from the dichloromethane extract of common purslane (Portulaca oleracea L.)

Date

4-2002

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn B. Rodriguez

Co-adviser

Maxima E. Flavier

Abstract

ABSTRACT

Nava, Anna Kathrina Panotes, University of the Philippines Los Banos. April 2002 Isolation and Characterization of Allelochemicals from the Dichloromethane Extract of Common Purslane Portulaca

Adviser Dr. Evelyn B. Rodriguez Co-adviser Dr. Maxima E. Flavier

The leaves, flowers, seeds and stems of mature common purslane (Portulaca oleracea L.), were sequentially extracted with ethanol and dichloromethane (CH2Cl2). The crude CH2Cl2 extract was bioassayed for inhibitory effect on radish (Raphanus sativus L.) seed germination. The crude CH2Cl2 extract was found to inhibit germination up to 51.11% at 333 ppm.

Column chromatography on silica gel of the crude CH2Cl2 extract yielded several fractions. Purification by recystallization resulted in five isolates, Allelo 1 to 5 Allelo 3, 4 and 5 inhibited radish seed germination.

IR and UV analyses of the isolated allelochemicals indicated that they are probably coumarins and flavonoids with -OH, and NH2 as possible substituents.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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