Isolation and partial characterization of a lectin from the seeds of Artocarpus camansi Blanco

Date

3-2004

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Florinia E. Merca

Abstract

ABSTRACT

OCCEÑA, IRVING V. University of the Philippines Los Banns, March 2004. Isolation and Partial Characterization of a Lectin from the Seeds of Artocarpus cumansi Blanco.

Adviser: Dr. Florinia E. Merca

A lectin was isolated and purified from the seeds of Artocarpus camansi Blanco by extraction with phosphate buffered saline at pH 7.2. Further purification was done by precipitation with ammonium sulfate at 0-90% saturation. The dialyzed ammonium sulfate precipitate was subjected to gel filtration chromatography using Sephadex G-200 and fractions were tested for lectin activity. Native PAGE was employed to establish the homogeneity of the lectin positive fractions. Fractions with the same band were pooled and lyophilized. The purified lectin is considered to be non-blood type specific since it agglutinated all human blood types. It was observed to be blood group specific because it agglutinated blood from cow but not from swine. The purified lectin is a complete lectin since it agglutinated erythrocytes without trypsin treatment and addition of metal ions. However, trypsination of erythrocytes increases the agglutination activity. The isolated lectin did not show any specificity towards the ten sugars used in this study. The molecular weight of the purified lectin was estimated by using Sephadex G-200 and was found to be around 2,000 kD since it eluted in the void volume. The lectin was found to be stable at pH 6-8 and 20-40"C.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2004 A13 O23

Document Type

Thesis

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