Local production of U.S.P grade dextrose from corn starch and cassava starch for intravenous (I.V) fluids via enzymatic hydrolysis using alpha-amylase and glucoamylase

Date

10-2003

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita M. Espino

Co-adviser

Nieva T. Librojo-Basilio

Abstract

ABSTRACT

OLAYRES, PAUL ARJUNE I P., College of Arts and Sciences, University of the Philippines Los Banos, October 2003. Local Production of U.S.P. Grade Dextrose from Corn Starch and Cassava Starch for Intravenous (I.V.) fluids via Enzymatic Hydrolysis using Alpha-amylase and Glucoamylase.

Adviser: DR. TERESITA M. ESPINO

United States Pharmacopoeia (U.S.P.) grade dextrose was produced via batch-scale enzymatic hydrolysis of cassava and corn starches as substrates with alpha-amylase (3287.17 DUN/mL) and glucoamylase (70.02 U/mL). Optimized conditions previously obtained by Espino et al. (2000) were used to carry out the process. The overall yields for dextrose monohydrate production were 94.22% (w/w) and 92.06% (w/w), while percent purities were 89.48% and 91.50% for cassava and corn starches, respectively.

Hydrolysis of either cassava or corn starch involved a series of liquefaction, saccharification, purification, refining and recrystallization steps that ensure the quality of the produced dextrose monohydrates. Physico-chemical and microbiological analyses of the dextrose monohydrate showed that majority of the tests complied with the USP set of standards in terms of identification test for glucose, odor, taste, color of solution, melting point, moisture content, residue on ignition, absence of dextrins and soluble starch, lead content for corn-derived dextrose, pH of 5% w/v solution, 10 1.1M particulate matter count, absence of hydroxymethylfurfural and its derivatives, microbial limit, and absence of toxins. Assays that showed deviation from the limit set by USP included solubility, specific rotation, acidity, chloride and sulfate contents, 25 um particulate matter count, and lead content for cassava-derived dextrose.

The enzymatic hydrolysis of cassava and corn starches using BIOTECH-produced enzymes provides an alternative method towards local production of dextrose monohydrate for parenteral purposes.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2003 C4 O43

Document Type

Thesis

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