Application of pectinase on the extraction of essential oils from lagundi (Vitex agnus castus)

Date

4-2007

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita M. Espino

Abstract

ABSTRACT

Olivarez, Nina Irene A., College of Arts and Sciences, University of the Philippines Los Banos. Application of Pectinase on the Extraction of Essential Oils from Lagundi (Vitex agnus castus).

Adviser: Dr. Teresita M. Espino

A study on the use of pectic enzymes for the extraction of essential oils from fresh Lagundi (Vitex agnus castus) leaves was conducted. The optimized conditions obtained for maximum oil yield from fresh lagundi leaves were as follows: enzyme concentration = 3.00 (w/v)%, incubation time = 24, sample:buffer dilution ratio = 1:4 (w/v). Enzymatic extraction of oil from fresh Lagundi leaves using the optimized conditions was compared with hydro steam distillation. Data showed that pectinase treatment resulted in 1.99% lagundi oil yield as compared to steam distillation which was 0.047% representing a 39.8 fold increase using the former method. Gas chromatographic analyses of the fatty acids of lagundi oil showed the presence of C16, palmitic acid.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2007 C4 O45

Document Type

Thesis

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