Isolation of water soluble galactomannan from "makapuno" (Cocos nucifera, Linn) and evaluation of angiogenic activity

Date

4-2008

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Maxima E. Flavier

Abstract

OÑA, JAY PEE PERMEJO. College of Arts and Sciences, University of the Philippines Los Banos. April 2008. Isolation of Water Soluble Galactomannan from "Makapuno" (Cocos nucifera, Linn.) and Evaluation of Angiogenic Activity.

ADVISER: Maxima E. Flavier Ph.D.

A water soluble galactomannan was isolated from the solid edosperm of makapuno (Cocos nucifera, L.) by exhaustive water extraction and precipitation with 70% ethanol. The molecular weight of the polysaccharide was determined by size exclusion chromatography and viscometry. The functional groups present were identified from infrared spectral analysis. Carbohydrate analysis was done by determining total sugars, total reducing sugars and monosaccharide composition. The polysaccharide was then evaluated for angiogenic and antimicrobial activities.

Molecular weight determination using size exclusion chromatography revealed that the major fraction has a molecular weight greater than 235,000 Dalton. Viscometry of the galactomannan solution showed an intrinsic viscosity of 3.7136 dl/g corresponding to a molecular weight of 331,000 Dalton. Infrared spectral analysis makapuno galactomannan showed the presence of hydroxyl (-OH), carbonyl (C=0) and uronic acids. Carbohydrate analysis showed that the isolate contained 73.06 % total sugars, 13.10 % reducing sugars and 59.96 % non-reducing sugars. Monosaccharide analysis using gas chromatography showed the presence of D-mannose and D-galactose in a ratio of 2.35:1.

Evaluation of the angiogenic activity using the duck embryo assay showed that the galactomannan-treated embryos had an increased formation of blood vessels compared to the untreated embryos. The polysaccharide exhibited no antimicrobial activity having zero zone of inhibition on a culture of methicillin-resistant Staphylococcus aureus (MRSA).

It was evident that the makapuno solid endosperm is a good source of galactomannan having a maximum yield of 57.46% (based on dry weight of the sample). The isolated polysaccharide was also found to be pro-angiogenic which may give insight to its immunostimulatory property. Pro-angiogenic compounds are needed in wound healing and helpful in minimizing tissue damage from stroke or heart attack.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2008 C4 O53

Document Type

Thesis

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