Isolation, purification and partial characterization of a lectin from the leaves of Pithecellobium dulce (Roxb.) Benth

Date

10-2000

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Marivic S. Lacsamana

Abstract

Leaves of fourteen locally available medicinal plants were surveyed for lectin activity. Nine of these plant species namely Aleurites moluccana (L) wild (kangkong), Allium sativum L (Garlic), Allium cepa L (Onion), Asparagus officinalis L. (Asparagus) Ficus elastica Roxb ex. Hornem. (Balete) Hibiscus rosa-sinensis L (Gumamela), Ipomea batatas (L) Lam. (Kamote) Pithecellobium dulce (Roxb) Benth (Kamachile) and Syzygium cumini (L) Skeels (Duhat) tested positive for the presence of lectin

The lectin detected in the leaves of P. dulce was isolated using extraction with 0.02 M phosphate buffered saline (pH 7.2)and purified using ammonium sulfate fractionation and gel permeation chromatography on sephadex G-200

The lectin is non-blood type and non-blood group specific because it agglutinated all human blood types (A,B, AB, and O) as well as animal erythrocytes such as those from calf, carabao, rabbit and swine. It can also be classified as a complete lectin since it can agglutinate erythrocytes in physiological saline solution without trypsination .

Hemagglutination by the lectin was inhibited by D(+) - glucose and D(+)-xylose at 250 mM using blood type B and by D(+)-glucose L(+)-arabinose and D(+)-xylose using blood type O at 500 mM.

The molecular weight of the purified lectin was estimated to be 79 KD using gel permeation chromatography on Sephadex G-200 Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed two protein bands with molecular weights of 66 KD ad 45 KD. The lectin is a glycoprotein containing 18.9% by weight total sugars as determined by the phenol-sulfuric acid method.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1990 A13 O64

Document Type

Thesis

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