A rapid methodology for the analysis of chlorinated insecticide residues in milk

Date

10-1974

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Carlito R. Barril

Abstract

A rapid operation and clean up technique for the residue analysis of milk was tested and compared with the official AOAC method and aboved very low recoveries. Modification of the method by substituting a one-step mini-column clean up technique for the liquid-liquid partitioning clean up step resulted not only to complete recovery of eleven chlorinated insecticides from spiked samples, but also to a simpler and more economical method.

Using the modified extraction and clean up technique, 18 commercial milk samples and human milk sample were analyzed for chlorinated insecticides residues by ECD gas chromatography. The results showed the general presence of chlorinated insecticides residues with levels ranging from trace amounts to as high as 82 ppb on whole milk basis, with a mean total DDT concentration of 69 ppb. All milk samples analyzed contained DDT and/or its metabolites DDE and DDD, reflecting the widespread environmental distribution of DDT as well as its long biological retention. Aside from the DDR group, other residues detected were aldrin, chlordane, dieldrin, endrin, heptachlor, heptachlor epoxide and lindane the number of residues present in a sample ranged from three to nine. The results also demonstrated the stability of chlorinated insecticides during processing since powdered milk samples analyzed contained residues.

The significant of the results as well as the possible sources of contamination of the milk dismissed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1974 A13 O73

Document Type

Thesis

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