Biosorption of cadmium ions from aqueous solutions by immobilized Rhizobium BJVR12 cells

Date

3-1994

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Juanita C. Mamaril

Co-adviser

Lilia S.P. Madamba

Abstract

The ability of the polysaccharides produced by Rhizobium BJVr12 cells to sequester cadmium ions was investigated. Different factor effects in relation to the amount and rates of cadmium sequestration were studied using an improvised column with the eluates analyzed through AAS.

Initially, the growth curve of Rhizobium BJVr12 was determined. Its general behavior was observed daily within a period of 10 days by measuring the average cell dry weight, average polysaccharide dry weight and average number of cellsimL per day. Maximum growth and polysaccharide production was found to occur at the 6th day after inoculation in a yeast-mannitol broth.

Three methods were utilized to observe the biosorption of cadmium ions. The results from the experiments of the cells imbibed into ceramic beads and of non-immobilized cells only using a shaker at different time periods showed low percentage adsorption. No significant differences between the concentration and time factors were found.

The third method utilized immobilized Rhizobium cells on carrageenan beads. 'the flow rate of 2mlJmin. showed lesser amount absorbed compared to a slower flow rate at 0.83mLimin. It was also observed that a greater concentration of Rhizobium cells immobilized in the beads absorbed greater amount of cadmium. Waste water experiments show that the biosorptive process is not selective to cadmium only.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1994 C4 P33

Document Type

Thesis

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