Screening of some Philippine medicinal plants for cytotoxic activity

Date

10-1995

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evamarie P. CaparedaLea

Abstract

Leaves from ten Philippines medicinal plants (avocado, banaba, alagaw, kakawati, guava, cacao, caimito, kape, manga, and santol) were collected from San Pablo City. The samples were extracted sequentially using four solvents of increasing polarity, petroleum ether (PR), dichloromethane (DCM), ethyl acetate (EIOAc) and methanol (MeOH). All the crude extracts were tested for cytotoxic activity using the brine shrimp (Artemia Selina) lethality assay.

The dichloromethane extracts of avocado (Persea americane), banaba (Legerstroemia speciousa) and kakawati (Gliricidia sepium) showed high average percent mortality at 0.01 mg/ml of 100, 80 and 85, respectively after 24 hours. The student focused on the dichloromethane extract of avocado (Persea Americana) because of its relatively strong lethality against the brine shrimp larvae. Fractionation of this extract by chromatographic methods resulted in the isolation of an active fraction that gave a single spot on TLC.

IR and UV data of this fraction suggest that the possible fractional groups presents are hydroxyl group, an aromatic ring and an ester.

Statistical analysis of the data showed that there were significant effects on the mortality of the brine shrimp larvae due to plant, solvent and time at 5% level of significance.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1995 C4 P38

Document Type

Thesis

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