Protection and efficient delivery of ferulic acid through rice bran phospholipid-based nanoliposomes

Date

4-2011

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn B. Rodriguez

Abstract

PAZ, JONATHAN VIRAY. College of Arts and Sciences. University of the Philippines Los Banos. March 2011. Protection and Efficient Delivery of Ferulic Acid through Rice Bran Phospholipid-Based Nanoliposomes

ADVISER: Evelyn B. Rodriguez, Ph.D.

Ferulic acid was nanoencapsulated using rice bran phospholipids in order to improve its bioavailability and delivery.

Phospholipids from rice bran were extracted using ethanol/n-hexane/water (16:10:4) and then purified by precipitating with acetone, to give a yield of 4.61 ± 0.36%. Using the ascorbic acid method, total phosphorus content of the phospholipids was 57.41 ± 0.36 mg P/100 g. HPTLC and densitometric analyses identified and quantified phosphatidylcholine (45.47%), phosphatidylethanolamine (12.17%) and lysophosphatidylethanolamine (26.10%) as major phospholipid components. GC analysis of fatty acids from the phospholipids identified and quantified linoleic acid (35.7 . 0.16 %). palmitic acid (28.6 ± 0.06 %) and oleic acid (26.7 ± 0.13 %) as major fatty acids.

The thin film method coupled with bath sonication was used in the preparation of ferulic acid nanoliposomes; the yield was 90.89 ± 6.10%. An encapsulation efficiency of 77.53 ± 2.03% was determined by a spectrophotometric method. Transmission electron microscopy (TEM) showed that the size of the nanoliposomes ranged from 129.25 to 166.19 nm with an average of 147.72 ± 18.47 nm. The in vitro releasing patterns in simulated gastric and intestinal fluids showed that only 17.46 ± 0.52 % of encapsulated ferulic acid was released from the nanoliposomes in the simulated gastric fluid over a 4-hour period while 50.96 ± 1.62 % of encapsulated ferulic acid was released from the nanoliposomes in simulated intestinal fluid for the same duration.

Free-radical and tyrosinase inhibiting activities of encapsulated and non-encapsulated ferulic acid were determined. Results of the DPPH assay in physiological conditions showed an increase in the free-radical scavenging activity of encapsulated ferulic acid (SC50 8.02 ± 0.81ppm) compared to non-encapsulated ferulic acid (SC50 26.85 t 1.25 ppm). The tyrosinase inhibiting activity of the encapsulated ferulic acid was found to be higher (IC50 6.11 ± 0.03 liM) than those of non-encapsulated ferulic acid (IC50 12.39 f 0.20 gM) and hydroquinone (IC50 8.83 ± 0.07 IA.M). Photodegradation studies showed that non-encapsulated ferulic acid was degraded by 50.27 ± 2.10 % after exposure to UV radiation (366 nm) for 2 hours, on the other hand, the degradation of encapsulated ferulic acid was only 14.71 f 1.90 % under the same conditions. These results indicated that encapsulation protected ferulic acid against UV damage.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2011 C4 P39

Document Type

Thesis

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