Physico-chemical characterization of the essential oil of Pittosporum resiniferum Hemsl. (petroleum nut) and its antibacterial activity against Staphylococcus epidermidis and Staphylococcus aureus

Date

4-2007

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita M. Espino

Co-adviser

Maxima E. Flavier

Abstract

PERALTA, PEARL MARIAN A. University of the Philippines Los Banos, April 2007. Physico-chemical Characterization of the Essential Oil of Pittosporum resiniferum Hemsl. and Its Antibacterial Activity against Staphylococcus epidermidis and Staphylococcus aureus,

Adviser: Teresita M. Espino Ph. D.

Pittosporum resiniferum Hemsl. otherwise known as petroleum nut or in the local language, Mange or "yanga" or "Tagong ngirit" fruit is a possible source of oil that can be used as biodiesel and at the same time as an antibacterial agent for topical applications on skin diseases caused by Staphylococcus epidermidis and Staphylococcus aureus. The essential oil from petroleum nut was obtained by hydro-steam distillation. Analysis of the physical properties of the petroleum nut oil showed that the oil had gasoline or petroleum —like smell, colorless, and produced sooty flame. The specific gravity of the oil was 0.819 while the peroxide value was 17. 256 and the iodine number was 108.87.

The antibacterial activity was tested to determine the inhibitory effects of the said oil against Staphylococcus epidermidis and Staphylococcus aureus. Broth dilution technique was used to determine the effect of the essential oil against the test bacteria. Results showed that the "Tagong Ngirit" essential oil (TNEO) concentrations ranging from 10 ppm to 500 ppm had antimicrobial activity against Staphylococcus epidermidis. On the other hand, higher concentrations of TNEO ranging from 400 to 500 ppm were needed to control growth of Staphylococcus aureus.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2007 C4 P47

Document Type

Thesis

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