Partial purification and characterization of pectinases of Aspergillus ficuum isolated from pomelo

Date

9-1987

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Carlito R. Barril

Abstract

PERONCE, RONNEL S. College of Agriculture - College of Arts and Sciences, University of the Philippines at Los Banos. September 1987. Protein Enrichment of Corn Cobs Through Microbial Fermentation Under Solid-State Conditions.

Adviser : Dr. Carlito R. Barril

The fungal fermentation using Chaetomium cellulolyticum and Chaetomium globosum of alkali (1% w/v Na011) pretreated corn cobs was investigated under solid-state conditions. Protein content of corn cobs was increased by more than three times its original amount. Shaking the fermentation vessel as well as using a bigger container increased the rate of microbial growth as reflected in the protein content relative to the time of fermentation. Corn cobs inoculated with C. cellulolyticum in 250 ml. flasks showed maximum protein production of 7.28% and 6.49% for the disturbed and the undisturbed cultures, which peaked on the fifth and sixth day, respectively; lengthening of the fermentation period failed to increase the protein content. Cultures grown in gallon jars (3.8 li. cap.) showed maximum protein .content of 8.31% after three days of fermentation using C. cellulolyticum. A lower maximum protein content of 7.88% after five days was obtained from corn cobs inoculated with C. globosum also grown in gallon jars.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1987 A13 P47

Document Type

Thesis

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