Isolation and partial characterization of an antibacterial compound from Cymbopogon citratus (DC) Stapf

Date

4-2002

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evamarie P. Capareda

Abstract

PIDELO, MARIA CARMENCITA A. University of the Philippines Los Bafios, Bachelor of Science in Chemistry, April 2002. Isolation and Partial Characterization of an Antibacterial Compound from Cymbopogon citratus (DC) Stapf.

Adviser: Dr. Evamarie P. Capareda

Dried, ground leaves of Cymbopogon citratus were extracted with solvents of increasing polarity: petroleum ether, dichloromethane, methanol then water. The antibacterial (agar-plate diffusion) assay-guided chromatographic separation of the petroleum ether extract led to the isolation of a colorless compound, D5, which upon exposure to light turned into a white opaque emulsion in chloroform. At 1000 ppm, D5 was found to have antibacterial activity against Staphylococcus aureus . The crude extract also exhibited inhibition of growth of the fungus, Sclerotium rolfsii. However, results of the antifungal assay were not promising after successive fractionations of the extract. Based on the UV and IR spectra, isolate D5 has a benzene ring and an ester functional group.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2002 C4 P53

Document Type

Thesis

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