Reusability of immobilized lipase for monoacylglycerol production by alcoholysis of coconut oil

Date

3-2025

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Abstract

PINEDA, JOCELYN A. University of the Philippines at Los Banos. March 1999. Reusability of Immobilized Lipase for Monoacylglycerol Production by Alcoholysis of Coconut Oil.

Adviser: Dr. Eugenia Magpantay Castillo

Immobilized lipase from Rhizopus delemar with a protein content of 4.93 mg/mL and a specific activity of 22.47 U/mg was used to catalyze the production of monoacylglycerol (MAG) using alcoholysis process. The percentage MAG produced and the change in lipolytic activity (LA) were monitored to determine the reusability of the immobilized enzyme.

Results showed that highest amount of 67.57% MAG was obtained after the first use. A decrease of 63.77% on the amount of MAG was noted after the second use but remained constant (24.00%) after the third and fourth reuse. The enzyme was still active with a lipolytic activity of 39.62 U, using a shake flask system, indicating the enzyme stability toward repeated alcoholysis reaction.

Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were used to identify and quantitate the monoacylglycerol produced.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1999 C4 P56

Document Type

Thesis

This document is currently not available here.

Share

COinS