Aerobic and anaerobic degradation of phenol by Pseudomonas aeruginosa strain NH34B

Date

4-2005

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

James A. Villanueva

Abstract

Pseudomonas aeruginosa strain NH34B, an isolate from North Harbor !sediment. was found to degrade phenol as its sole carbon source under aerobic and denitrifying conditions. Different factors influence the extent of phenol degradation such as pH, shaking, and KNO3 concentration. Effective aerobic degradation was observed at 300 rpm as compared to those at 150 rpm. On the other hand, no denitrification took place at very acidic (pH 3) and basic (pH 9) environments. Samples with 0.5 g/L, 1.0 g/L, and 3.0 g/L KNO3 were more efficient in anaerobic degradation than those with 0.1 g/L and 0.3 g/L KNO3. However, from the data obtained, pH has no significant effects on aerobic degradation, and temperature does not affect anaerobic degradation. The growth under aerobic and anaerobic conditions showed a specific growth rate (u) of 1.047 hr-1 and 0.311 hr-1, respectively. The generation time (G) was calculated to be 0.662 hr and 2.23 hrs for aerobic and anaerobic, respectively. Apparent cellular Ks and Vmax were estimated to be 15.036 mM phenol and 8.280 mM phenol/day. The chromatograms and mass spectra generated from LC-MS analysis confirmed that catechol is a metabolite in the aerobic degradation of phenol, and 6-Hydroxycyclohex-1-ene-1-carboxyl-CoA or 6-0xo-2- hydroxycyclohexane-1-carboxyl-CoA are probable metabolites in anaerobic degradation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2005 C4 P67

Document Type

Thesis

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